Using Biosafety Cabinet (BSC)

BEFORE STARTING WORK

  • Wear gloves and a lab coat
  • Make sure UV lights are off
  • Ensure sash is in correct position
  • Check that air grilles are unobstructed
  • Make sure air is flowing inward
  • Disinfect all working surfaces

DURING WORK

  • Don’t move your hands and arms in and out of the BSC excessively
  • Keep contaminated material towards the back of the BSC
  • If you’ve used it in the BSC, throw it away in the BSC
  • Don’t use open flames

AFTER FINISHING WORK

  • Leave BSC unused and running for 5 minutes
  • Make sure all containers are closed before taking them out of the BSC
  • Disinfect all contaminated material with 70% ethanol or 1% NaClO (1:6 bleach solution) for 15 minutes before taking them out of the BSC
  • Disinfect all work surfaces in the BSC while it is still running
  • Remove and dispose of gloves in the BSC
  • Wash hands before leaving the lab