CAGEF provides typing services for isolated bacteria using Multi-Locus Sequence Typing (MLST), 16S rRNA, and species-specific gene sequencing. We use high-throughput Sanger and Next-Gen targeted amplicon sequencing to determine variants and compare these against the species-approriate pubMLST database or against available gene sequences to determine relationships. Besides providing the resulting sequences in FASTA format, we offer phylogenetic and eBURST network analyses.
For typing, we require a minimum of 300ng of total DNA, at a minimum concentration of 20ng/μl. The OD260/280 for all samples should be at least 1.8.